@prefix this: <http://purl.org/np/RA8LXJxDVioLwvnT7U0GREq_szXgcjzp3WYAyInmPlWNQ> .
@prefix sub: <http://purl.org/np/RA8LXJxDVioLwvnT7U0GREq_szXgcjzp3WYAyInmPlWNQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0400155> rdfs:label "water bath" .
  
  sub:_1 rdfs:label "lysozyme solution" .
  
  sub:step dc:description """Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""";
    a p-plan:Step;
    bs:bioSampleUsed <http://www.bioassayontology.org/bao#BAO_0002104>;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400155>;
    bs:reagentUsed sub:_1 .
  
  <http://www.bioassayontology.org/bao#BAO_0002104> rdfs:label "suspension" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-26T09:44:15.524571"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:Mm4SaHeyEeupA9_TrHGoQQ, authority:SxYACneyEeuhZweY7act7g,
      authority:f0Q7MHeyEeum_5cx2E1wjg, authority:osRxYHeyEeu5zVuedDPw0Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "fHtQyp6CR1dzknNYSulii53Kpdx63g3ELHhARErrwVgcSxWDhvAeQhEPO6hAGppZitcTqNsyFZd9SXgrBy11gVFRB7ghF97zELzr5YgycWfkPd+ggsnjOS/EvFa/Dyx3R6eRdNOV36npp0akvuhqvFSDIer7hkd5VxmNTm08B7k=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-26T09:44:15.524571"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}