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<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://www.nanopub.org/nschema#hasProvenance> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#provenance> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#Head> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#Head> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#Head> .
<http://purl.obolibrary.org/obo/CHEBI_17824> <http://www.w3.org/2000/01/rdf-schema#label> "isopropanol" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#_1> <http://www.w3.org/2000/01/rdf-schema#label> "supernatant" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <http://purl.org/dc/elements/1.1/description> "Transfer the very clear supernatant to a fresh tube , add two fold volume cold  isopropanol and keep it for 1 hour at -20º celcius  ." <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#_1> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://www.bioassayontology.org/bao#BAO_0010014> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.obolibrary.org/obo/CHEBI_17824> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://www.bioassayontology.org/bao#BAO_0010014> <http://www.w3.org/2000/01/rdf-schema#label> "tube" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#provenance> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-02-24T16:00:10.311549"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#provenance> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/zuluaga.lizzz> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#provenance> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/TGxBcHbhEeuuMJ_WA7wCow> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/ZV9P9nbhEeuygj9TEkazIQ> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/eazBtHbhEeugrM80dicxjg> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/kLU9qnbhEeuiCHOB9H3qnw> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#sig> <http://purl.org/nanopub/x/hasPublicKey> "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#sig> <http://purl.org/nanopub/x/hasSignature> "OiUAGU1DkV9qdnFlvWOF+pxUDh7xorZnCs9wDHKV49R/IJzey6gIjIlp9XGBkLJCz3WUvHIAugGQK0SX4sdkv1ScGInIdYS+q9R0FZ+jbvxAnss4LkHd/fW+ZhmZ8hhfTTYtqsMeGYg/5KnUPUJVl+GcleoEs0DcjLmfROCWFQ8=" <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#step> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://www.w3.org/ns/prov#generatedAtTime> "2021-02-24T16:00:10.311549"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .
<http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/zuluaga.lizzz> <http://purl.org/np/RA83hcBONWf3SlTIBjZxSDKssKk83p1LmBLLGd6ntZI08#pubInfo> .