http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#Head http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.nanopub.org/nschema#hasAssertion http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.nanopub.org/nschema#hasProvenance http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#provenance http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.nanopub.org/nschema#hasPublicationInfo http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#pubInfo http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#_1 http://www.w3.org/2000/01/rdf-schema#label cell suspension http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#_2 http://www.w3.org/2000/01/rdf-schema#label 24 well culture plate http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step http://purl.org/dc/elements/1.1/description Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates. http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/net/p-plan#Step http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#_1 http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#_2 http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#provenance http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://purl.org/pav/createdWith https://nanotate.bitsfetch.com http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://www.w3.org/ns/prov#generatedAtTime 2021-02-14T05:25:31.936259 http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/zuluaga.lizzz http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#pubInfo http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/V-kpfG6uEeunoAOTPgQJFQ http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/ba-t2m6uEeucdPvQv1fpyQ http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#assertion http://www.w3.org/ns/prov#wasDerivedFrom https://hypothes.is/a/hL_d4m6uEeu2HOeqlNADQQ http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#sig http://purl.org/nanopub/x/hasAlgorithm RSA http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#sig http://purl.org/nanopub/x/hasPublicKey MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#sig http://purl.org/nanopub/x/hasSignature Ch1XrzNj8/n2Mc6yIB9Fai8SHUGy3PrCF6yLDxK6pGSXqqzy3euLyc/18I3YRXluHBYYfuLBOXymd4ifAoc400tey7BpGghyjOmzcHB6b1JtMrJhB+9p2di0t33ZUZxdKY24QlWcXLofyFF+zHamD4uByv2I0ZpSVmVYMdurr/w= http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#sig http://purl.org/nanopub/x/hasSignatureTarget http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://purl.org/nanopub/x/introduces http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.w3.org/ns/prov#generatedAtTime 2021-02-14T05:25:31.936259 http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE http://www.w3.org/ns/prov#wasAttributedTo https://hypothes.is/users/zuluaga.lizzz