http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#Head
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.nanopub.org/nschema#hasAssertion
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.nanopub.org/nschema#hasProvenance
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#provenance
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.nanopub.org/nschema#hasPublicationInfo
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#pubInfo
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://www.nanopub.org/nschema#Nanopublication
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://purl.obolibrary.org/obo/OBI_0002488
http://www.w3.org/2000/01/rdf-schema#label
pipette
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_1
http://www.w3.org/2000/01/rdf-schema#label
mycobacteria
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_2
http://www.w3.org/2000/01/rdf-schema#label
mycobacteria
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_3
http://www.w3.org/2000/01/rdf-schema#label
log phase culture
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_4
http://www.w3.org/2000/01/rdf-schema#label
TE
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
http://purl.org/dc/elements/1.1/description
Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette.
Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_1
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://purl.org/net/p-plan#Step
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_2
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://purl.obolibrary.org/obo/OBI_0002488
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_3
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#_4
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#provenance
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://purl.org/pav/createdWith
https://nanotate.bitsfetch.com
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#generatedAtTime
2021-02-26T09:45:59.609387
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#pubInfo
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/Omq7ZnexEeueAA-4Ziqxeg
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/fpY55nexEeuGACeYmzK6Tw
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/mSDgSnexEeuhY99_AxTSHA
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/q_iw0HexEeu8DDM9AtM47w
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/zKzFyHexEeu14yM5lwg2aA
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#sig
http://purl.org/nanopub/x/hasAlgorithm
RSA
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#sig
http://purl.org/nanopub/x/hasPublicKey
MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#sig
http://purl.org/nanopub/x/hasSignature
VuLlQnlwr/b3r9gKLFepvxdP/9l1lLHIQc6DvAmlTsrDuRMiS5ziKhRmRlYZBTXS+aJVCOgEwl1c3Wadno5aVWqGXJs27W46ITDK/shlepXJ6bimA/NRQ/0sjRJqT+F+ctf+dCEEqetRwGLJeO2stB/0AyYRrSUix8Z3QkiItdM=
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#sig
http://purl.org/nanopub/x/hasSignatureTarget
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://purl.org/nanopub/x/introduces
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#step
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.w3.org/ns/prov#generatedAtTime
2021-02-26T09:45:59.609387
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz