@prefix this: <http://purl.org/np/RA3YD3Pt8TUVEX9idVVTqPkqjN8Pqe0j2KkUhBFOYt0uM> .
@prefix sub: <http://purl.org/np/RA3YD3Pt8TUVEX9idVVTqPkqjN8Pqe0j2KkUhBFOYt0uM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix p-plan: <http://purl.org/net/p-plan#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/PO_0025034> rdfs:label "leaf tissues" .
  
  sub:_1 rdfs:label "extraction buffer" .
  
  sub:step dc:description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/PO_0025034>;
    a p-plan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/PO_0025034>;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed sub:_1 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "50 mL Falcon tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-11T16:45:43.287006"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:6TwtXIKuEeufVs8lGVSnJg, authority:_rye-oKuEeuS7bc4SYlicg,
      authority:quasvIKuEeuXbCtzLANu_g, authority:zpbWHoKuEeujimtnDxFfPQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "AlkRqY3aHl5OD/+YmByHNB4OgJ5aSDjD360S8i0agflM75D5aBH8zIjAZn0ijQTrKYoX1cNWeqaoVpOZ3zbNZCa3jxVRzcdMx1HjR0dnaBSNERs+vLHYTpMEUeIdqzyOv1MXBESeVkVinH+CrJLEwxAkBitb7Sz3d4UiHLS2BkU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-11T16:45:43.287006"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}