. . . . "100mM sucrose" . "1% Triton -X" . "1% SDS" . "mosquitoes" . "mosquitoes" . "micropistle" . "Rnase A (20mg/ml)" . "1X STE buffer" . "Proteinase K (20mg/ml)" . "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." . . . . . . . . . . . . "1.5 ml eppendorf tube" . . "2021-03-16T16:48:04.108013"^^ . . . . . . . . . . . . "RSA" . "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" . "aZd/AL+wAll/02gNVAmhv8QGPBCTs7otfD4kaMz6CzndlDewoaVZcCmCBsl0xltZZZisvKm9bu+mhYyIwYX+5z4L3rxcyLp7AxPMD7roKQ7W9/5gRzY5DSAJH9kE9Y6jZKoTkDGwyXty1qOddP/83ut8S3q0w1HrYrcGVrpP6OU=" . . . "2021-03-16T16:48:04.108013"^^ . .