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hiPSCs that were seeded as single cells or small clumps of ~3 cells formed hollow, lumenoid, structures after 4 days of culture, as verified by LLS imaging.
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Tobias Kuhn
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WTC-11 cells form lumenoids within 3 days when grown in an extracellular matrix gel such as matrigel at several different concentrations (2 to 10 parts matrigel to 1 part cells) - by Tobias Kuhn