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<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://www.nanopub.org/nschema#hasProvenance> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#provenance> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#Head> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#Head> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#Head> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_1> <http://www.w3.org/2000/01/rdf-schema#label> "clear supernatant" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_2> <http://www.w3.org/2000/01/rdf-schema#label> "5 M NaCl " <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_3> <http://www.w3.org/2000/01/rdf-schema#label> "RNAse A" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_4> <http://www.w3.org/2000/01/rdf-schema#label> "chloroform: isoamyl alcohol" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <http://purl.org/dc/elements/1.1/description> "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_1> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://www.bioassayontology.org/bao#BAO_0010014> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_2> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_3> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#_4> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://www.bioassayontology.org/bao#BAO_0010014> <http://www.w3.org/2000/01/rdf-schema#label> "tube" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#provenance> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-18T16:12:45.992754"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#provenance> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#provenance> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/8tPhFoFLEeugr5u2BPA1Kw> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/GghLeIFMEeu23ZtkPfVRMA> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/P5lxUIFMEeuVocP4Xt_PMA> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/ZrwoGIFMEeu00ofuLN-6KA> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/hQCEroFMEeuvmA-YJowmfw> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/p826BoFMEeuUHBMr8wtiOw> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#sig> <http://purl.org/nanopub/x/hasPublicKey> "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#sig> <http://purl.org/nanopub/x/hasSignature> "Dn57705sr6nfnUYv+feGvEq8fF1KTTSxqz8CYrslt2NPDHQa3I8Emp/dOCcuU/f2yyeJoNBJzzY1zoAnC6XUE3pwtaPG1fhMgPQ3bb3MWs5NsPUQBhmrtWJTj9cUFcn8IRcXOjsc+dB8Tbg31OyTCkI4lZwNHJ+GHmeGiHLQ0Aw=" <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#step> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-18T16:12:45.992754"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .
<http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RA-zcNNUu2iaebpQ33_cARpsxM_kdFkp_BJK0rqnvoMRE#pubInfo> .